fluorescence cy3 labeled goat anti rabbit igg  (Boster Bio)

Journal: Biology direct

Article Title: Inhibition of RAC1 activator DOCK2 ameliorates cholestatic liver injury via regulating macrophage polarisation and hepatic stellate cell activation.

doi: 10.1186/s13062-025-00612-3

Figure Lengend Snippet: Fig. 5 DOCK2 activates RAC1 to participate in BDL-induced liver injury and its deficiency suppressed LPS-induced M1 macrophage polarisation. (A and B) Representative microphotographs of double IF staining of DOCK2 and RAC1 in 3d and 2w BDL livers. Magnification: 600 fold. Scale bar: 50 μm. (C and D) Representative microphotographs of IF staining of GTP-RAC1 in 3d and 2w BDL livers. The quantification of mean fluorescence intensity (MFI) is shown in the right panels. Magnification: 400 fold. Scale bar: 50 μm

Article Snippet: The slices were then incubated with the corresponding horseradish peroxidase-conjugated (Solarbio; Cat. #: SE134, RRID: AB_2797593) or fluorescence dye-labelled secondary antibodies (Proteintech; Cat. #: SA00009-2 and SA000031, RRID: AB_2890957 and RRID: AB_2890896) and photographed at 200, 400 or 600 magnification.

Techniques: Staining, Fluorescence

Journal: Biology direct

Article Title: Inhibition of RAC1 activator DOCK2 ameliorates cholestatic liver injury via regulating macrophage polarisation and hepatic stellate cell activation.

doi: 10.1186/s13062-025-00612-3

Figure Lengend Snippet: Fig. 6 Knockdown of DOCK2 suppressed LPS-induced M1 polarisation of mouse macrophages. (A) Schematic illustration of experimental protocols. (B) Protein levels of DOCK2 6 h after 100 ng/mL LPS stimulation. (C) Relative mRNA level of DOCK2 in RAW264.7 cells 48 h after adenovirus transduction. (D) 48 h after adenovirus transduction, cells were stimulated with 100 ng/mL LPS. Representative microphotographs of IF staining of iNOS 6 h later. Magnifica tion: 400 fold. Scale bar: 50 μm. (E) Relative mRNA levels of M1 macrophage cytokines (IL-6, TNF-α, and iNOS). (F) Representative microphotographs of IF staining of GTP-RAC1 in cells. Magnification: 400 fold. Scale bar: 50 μm. The quantification of mean fluorescence intensity (MFI) is shown in the right panel. (G) The pull-down assay of GTP-RAC1 by PAK-PBD protein beads. (H) Protein levels of DOCK2 in cells

Article Snippet: The slices were then incubated with the corresponding horseradish peroxidase-conjugated (Solarbio; Cat. #: SE134, RRID: AB_2797593) or fluorescence dye-labelled secondary antibodies (Proteintech; Cat. #: SA00009-2 and SA000031, RRID: AB_2890957 and RRID: AB_2890896) and photographed at 200, 400 or 600 magnification.

Techniques: Knockdown, Transduction, Staining, Fluorescence, Pull Down Assay